DETERMINATION OF MICROORGANISMS AND PH IN INDOOR DUST FROM CRUDE OIL IMPACTED COMMUNITIES IN OGONI, RIVERS STATE NIGERIA.

Author(s): Lucky .G.B; Boisa .N; and Konne .J.L1
Page: 29 - 38
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Publisher: The Creek Journals
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Keywords: Microorganisms, pH, Indoor dust

Abstract:

This study was designed to determine microorganisms and pH in Indoor Dust from six crude oil impacted Ogoni communities, which covers three Local government Areas in Ogoni. Dust samples were collected from five points in each location. For microorganisms analysis, the glassware were sterilised, the dust was prepared by means of serial dilution for the thirty (30) samples collected from the six locations. The media (Nutrient Agar (NA), Sabroud Dextrose Agar (SDA) were prepared and incubated for 24hours at 370C for the growth of bacteria and 48 hours for the growth of fungi and finally enumerated using a Digital Colony Counter and recorded. Test like Gram Staining, Motility Test and some Biochemical test were carried out on the Bacterial isolates for its Identification. The macroscopic and morphological characteristic of fungi isolates were carried out and a Catalase for Fungi Identification. For the pH analysis, the sample was prepared, the pH meter calibrated, dipped into the prepared sample and recorded. The result for Total Heterotrophic Bacteria indicated highest in Korokoro Tai with value 3.28cfu/ml, Bodo City 3.13cfu/ml, K-Dere 2.821cfu/ml, Kpean 2.19cfu/ml, Kwawa 2.09cfu/ml and the lowest value in Bori 2.01cfu/ml. For Total Fungi count Bori indicated the highest mean value 2.10cfu/ml, Korokoro Tai 1.99cfu/ml, Bodo city 1.99cfu/ml, Kpean 1.89cfu/ml, K-dere 1.80cfu/ml and the lowest at Kwawa 1.74cfu/ml. Bacteria like Pseudomonas was identified in Bodo city, K-Dere, Bori and Kwawa. Bacillus was identified in Bodo city, Korokoro Tai, K-Dere, Kpean and Kwawa Staphylococcus was identified in Bodo city, Bori, Korokoro Tai and Kpean Micrococcus was identified in Korokoro Tai. Fungi like Aspergillus flavus was identified in Bodo City and Kwawa Rhizopus stolonifera was identified in Bodo City, K-Dere, Kpean and Bori, Mucor was identified in Bodo City and Kwawa, Penicillium was identified in Korokoro Tai, K-Dere, Bori and Kwawa, Aspergillus niger was identified in Korokoro Tai, K-Dere and Bori. The mean pH values from Korokoro Tai 8.28±0.68, Bodo 7.75±0.17, Bori 7.44±0.13, Kwawa 7.22±0.04 Kpean 7.22±0.04 and K-Dere 7.16±0.21. The pH of dust in the study areas is alkaline. A lot of disease-causing bacteria and fungi were found in the dust samples collected from these communities. The built or residential environment indicated possible risk from indoor dust exposure. Ministry of Environment and other Agencies involved in the `on-going clean-up exercise in Ogoni should be aware of these findings and the people from these areas should be enlightened about these findings and encouraged to engage in regular cleaning of surfaces in homes to reduce possible exposure to disease associated with indoor dust.

Citations:

Lucky .G.B; Boisa .N; and Konne .J.L1 "DETERMINATION OF MICROORGANISMS AND PH IN INDOOR DUST FROM CRUDE OIL IMPACTED COMMUNITIES IN OGONI, RIVERS STATE NIGERIA." Volume 1 Issue 1 Oct, 2025